Lab Technician

Until is a moonshot company building a “pause button” for biology. Our near-term focus is organ-scale reversible cryopreservation: preserving donated organs at subzero temperatures without ice formation, then rewarming them uniformly for transplant. By solving this grand challenge, we’re laying the foundation for whole-body reversible cryopreservation, giving patients a bridge to future cures.

To achieve our goal, we are assembling an interdisciplinary team to develop perfusion systems, cryoprotectant formulations, and vitrification and rewarming hardware.

We envision a future where no transplantable organ is lost to logistics, and no terminal diagnosis is final because patients can safely wait for future medicine to arrive.

About the Role

Key Responsibilites:

• Express recombinant proteins in E. coli and yeast using established protocols, including culture preparation, growth, induction, and harvesting.
• Purify proteins using affinity chromatography, concentration, and buffer exchange.
• Assess protein concentration, yield, and purity using SDS-PAGE and protein concentration assays.
• Prepare and replenish culture media, buffers, stock solutions, and experimental reagents, including accurate weighing, dilution, pH adjustment, and sterilization as appropriate.
• After training, handle and maintain mammalian cells, including thawing, media changes, passaging, cell counting, viability assessment, and cryopreservation.
• Monitor cell morphology, confluency, and culture health; follow aseptic practices and promptly report contamination or unexpected results.
• Label, store, and track proteins, reagents, and cell stocks, maintaining clear records of preparation, production, quality control, and cell maintenance.

Required Qualifications:

• Bachelor’s degree in Biology, Biochemistry, Bioengineering, Molecular Biology, Chemistry, or a related field.
• At least two years of hands-on laboratory experience, including relevant academic research, internships, or industry experience.
• Hands-on experience with molecular biology and protein purification.
• Familiarity with SDS-PAGE, protein concentration assays, accurate pipetting, solution preparation, and routine laboratory calculations.
• Familiarity with aseptic technique, laboratory safety, and contamination-control practices.
• Strong organization, attention to detail, and documentation skills, with the ability to follow protocols, coordinate multiple tasks, and communicate deviations.

Preferred Qualifications:

• Experience expressing recombinant proteins in both E. coli and yeast.
• Experience with chromatography systems.
• Experience with larger-scale protein production and batch control.
• Experience with routine mammalian cell handling and maintenance.
• Candidates with strong protein-production experience who are interested in learning these techniques are encouraged to apply.

As an equal opportunity employer, Until is committed to providing employment opportunities to all individuals. All applicants for positions at Until will be treated without regard to race, color, ethnicity, religion, sex, gender, gender identity and expression, sexual orientation, national origin, disability, age, marital status, veteran status, pregnancy, or any other basis prohibited by applicable law.