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Open nowFirst seen 3 days ago

Cell Biologist

Globalchannelmanagement685 open roles

Where
Boston, Massachusetts, United States
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Your applicationOpen nowCell BiologistGlobalchannelmanagement · Boston, Massachusetts, United States

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The clock on this job

Early applications get read.

12.1% of postings close within 7 days. Measured by our own scanner across the market.

Share of postings closed within
  1. 2.1%1 day
  2. 4.5%3 days
  3. 12.1%7 days
  4. 19.1%14 days
  5. 35.6%30 days
  6. 52.7%60 days
This job: first seen 3 days ago

The posting

Cell Biologist needs 3+ years for MS - 5+ years for BS. Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques.

Cell Biologist requires:

  • Proficiency in gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting.
  • Hands-on experience with cell delivery methods of gene editing tools.
  • Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment.
  • Strong organizational skills and diligent record keeping.
  • Experience in pancreatic islet directed differentiation is highly desirable
  • Hands-on experience in transitioning research-grade processes to GMP-grade is highly desirable.
  • Mandatory weekend cell culture responsibilities on rotation basis.

Cell Biologist duties:

  • Enabling and advancing iPSC gene editing, cell line development and evaluation of differentiated cells.
  • Routine iPSC cell culture, passaging and media making.
  • Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening and delivery.
  • Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows.
  • Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR.
  • Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, metabolite assessments.
  • Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance.
  • Collaborate cross-functionally to support transfer of R&D assays to GMP.
  • Maintain accurate and detailed records of all experiments, synthesize results, prepare reports and present in meetings.
  • Contribute to SOP development.
  • Work seamlessly in executing workplans and meeting deadlines.
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