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Open nowPosted 17 days ago

Scientist, Neutrophil BIology, SIgN

MyCareersFuture94,028 open roles

Pay
SGD 4,750 – SGD 9,500 a month
Where
West, Singapore
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Your applicationOpen nowScientist, Neutrophil BIology, SIgNMyCareersFuture · West, Singapore

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This job: posted 17 days ago

The posting

The Lab of Immune Regulation, Resolution & Tolerance at Singapore Immunology Network (SIgN) is seeking a motivated and interdisciplinary postdoctoral scientist to join our team in the field of neutrophil biology. The successful candidate will lead projects that contribute to advancing our understanding of how neutrophil heterogeneity impacts inflammation resolution and tolerance. They will have access to cutting-edge sequencing and spatial transcriptomics techniques to dissect the role of neutrophil subsets in mouse models of inflammation and cancer, and subsequently apply their findings to human samples.

Requirements

Key Responsibilities:

Investigate how neutrophil reprogramming is spatially and temporally directed by the local environment in inflammation and cancer

  • Design, analyse and interpret single-cell RNAsequencing (scRNAseq) and spatial transcriptomics datasets, with the specific conservation of neutrophils.
  • Conduct in vivo mouse model experiments, including mouse handling, surgical procedures, end-point sample harvesting, ensuring compliance with regulatory and ethical requirements.
  • Conduct in vitro wet lab experiments, including cell culture of both cell lines and primary neutrophils, immunofluorescence staining, among others.
  • Design, perform and optimise a wide array of experiments using techniques such as flow cytometry, fluorescence activated cell sorting, CRISPR-based editing.
  • Handle and manage human clinical samples, ensuring compliance with regulatory and ethical requirements.
  • Analyse data using a combination of standard software including Flowjo (flow cytometry) and bioinformatics tools (R, Python).

Team contribution, mentoring and collaboration

  • Provide mentorship and guidance to students and junior researchers in the lab
  • Engage and work closely with multidisciplinary team of collaborators across Singapore
  • Work closely with the PI (Melissa Ng), to align research priorities and meet milestones

Job Requirements:

  • Ph.D. in Biomedical Sciences/Engineering, with an Immunology specialization strongly preferred.
  • Strong understanding of immunology and cancer biology, with a focus on neutrophil biology.
  • Strong track record of peer-reviewed publications appropriate to the current career stage
  • Experience in designing and executing experiments with a high degree of technical rigour. Familiarity with cell culture, primary immune cell isolation and flow cytometry is preferred.
  • Familiarity with next generation sequencing, especially for spatial transcriptomics and scRNAseq is a plus.
  • Excellent problem-solving and critical thinking skills with the ability to work independently and collaboratively within a team environment.
  • Strong written and oral communication skills, with experience in scientific publication and presentations

Why join us:

  • Be part of a young and dynamic new team advancing research into immune tolerance and resolution
  • Gain experience working at the intersection of wet and dry lab with access to state-of-the-art facilities and technologies
  • Perform rigorous science with strong translational capabilities
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